Kev xaiv thaj chaw membrane
Tom qab txiav txim siab qhov sib kis flux thiab ntim kev kho mob, thaj tsam ntawm lub lim membrane yuav tsum tau txiav txim siab. Lub cheeb tsam membrane yog xam raws li nram no:
Thaum xam qhov chaw ntawm daim nyias nyias nyob rau hauv cov txheej txheem scale-up, ib qho kev nyab xeeb feem ntau yog teem kom txo qhov kev cuam tshuam ntawm batch rau batch sib txawv ntawm cov txheej txheem tangential flow filtration. Raws li qhov xwm txheej ib txwm muaj, qhov kev nyab xeeb yog 1.2, thiab qhov kev nyab xeeb no tuaj yeem nce lossis txo ua ke nrog cov txheej txheem tiag tiag.
Raws li daim nyias nyias cheeb tsam xam mis, lub membrane cheeb tsam yog inversely proportional rau lub hom phiaj ua lub sij hawm. Qhov loj dua ntawm daim nyias nyias, lub sijhawm ua haujlwm luv dua; Qhov me me ntawm daim nyias nyias, lub sijhawm ua haujlwm ntev dua. Thaum xaiv qhov tsim nyog ntawm daim nyias nyias, nws tseem yuav tsum tau them sai sai rau cov khoom ntim ntawm daim nyias nyias. Kev ua haujlwm me me ntawm daim nyias nyias nrog cov kua dej ntau, uas yog, daim nyias nyias overload, yuav cuam tshuam rau lub neej kev pabcuam ntawm daim nyias nyias thiab txawm tias ua rau kev ncaj ncees ntawm daim nyias nyias.
Kev tu qhov system
Lub tangential flow ultrafiltration system yuav tsum tau ntxuav ua ntej thiab tom qab siv. Lub kaw lus muaj ntau cov tshuaj preservatives ua ntej siv, thiab lub kaw lus uas tsis siv rau lub sij hawm ntev yuav muaj ntau yam impurities. Tom qab siv, ntau yam kuab paug yuav nyob rau hauv lub system, uas ua rau txo cov membrane kev ua tau zoo thiab shortened membrane lub neej. Txhawm rau kom rov ua haujlwm ntawm lub lim membrane, tiv thaiv lub lim membrane los ntawm kev sib kis thiab thaiv, thiab tsis txhob hla kev sib kis ntawm cov khoom siv, kev ntxuav ua ntej thiab tom qab siv yog ib qho tseem ceeb heev ntawm kev saib xyuas txhua hnub.
Cov hauv qab no yog cov txheej txheem tu dav dav ntawm tangential flow ultrafiltration system:
Ua ntej siv:
Ntxuav: Yaug lub preservative. Cov dej ntshiab pom zoo yog 20-40L/m². Lub siab pom zoo rau kev ntxuav yog raws li hauv qab no.
Tom qab siv:
1. Ntxuav: ntxuav qhov chaw thiab sab hauv cov protein thiab impurities ntawm lub cev. Qhov pom zoo ntawm cov dej lim dej yog 20-40L / m². Nws raug nquahu kom ntxuav dej ua ntej siv nrog lub siab.
2. Kev ntxuav: tshem tawm cov impurities sab hauv, cov khoom, cov kab mob, lwm yam proteins, thiab lwm yam. Pom zoo siab raws li saum toj no. Cov hauv qab no feem ntau yog siv cov tshuaj ntxuav thiab cov khoom siv tu kom zoo.
3. Preservation: tom qab ntxuav yog ntxuav, thiab tom qab ntxuav, siv preservation kua cycle tu rau 10min, lub siab pom zoo yog tib yam li saum toj no. Cov hauv qab no yog cov kev daws teeb meem ntau thiab cov khoom khaws cia sib xws.
Membrane life verification
Nrog rau kev rov siv dua ntawm ultrafiltration membrane cassette, rov qab sib cuag nrog cov qauv yuav tsum tau kho thiab ntau yam buffers yuav ua rau kom txo qis lossis txiav tawm lub neej ntawm ultrafiltration membrane cassette. Lub attenuation ntawm lub neej ntawm ultrafiltration membrane cassette yuav cuam tshuam tus nqi ntawm tag nrho cov txheej txheem, thiab lub attenuation yuav ua rau tsis ua hauj lwm mus tas li thiab zoo raws li cov txheej txheem yuav tsum tau.
Kev txheeb xyuas lub neej ntawm daim nyias nyias yuav tsum simulate cov txheej txheem tsim khoom tiag tiag, ua qhov kev tshuaj xyuas ib ntus ntawm kev ntxuav, siv thiab khaws cia cov kauj ruam, thiab txheeb xyuas qhov ncaj ncees, dej ntws, sediment thiab cov qauv tawm los ntawm cov membrane cassette ntawm qee lub sijhawm, thiab txiav txim siab tus lej siab tshaj plaws. ntawm kev siv cov membrane cassette raws li cov ntaub ntawv pov thawj.
Cov hauv qab no yuav qhia txog kev ua haujlwm tshwj xeeb ntawm daim nyias nyias lub neej pov thawj raws li qee lub rooj dawb paug.
Encapsulation kev ntsuam xyuas kev ncaj ncees yog ib txoj hauv kev tseem ceeb los xyuas kom meej cov khoom zoo thiab kev nyab xeeb. Hauv kev lag luam biopharmaceutical, lub hnab ntawv kev ncaj ncees muaj feem cuam tshuam rau kev nyab xeeb thiab kev ua tau zoo. Thaum qhov kev ncaj ncees ntawm daim nyias nyias tsis yog raws li tus qauv, lub membrane yuav tsum tau hloov.
Kev pabcuam lub neej ntawm daim nyias nyias tuaj yeem txhim kho raws li kev saib xyuas txhua hnub, thiab kev tu kom zoo thiab siv tau zoo tom qab siv (cov dej ntws tsis pub tsawg dua 80% ntawm cov dej ntws thawj zaug) tuaj yeem txhim kho kev pabcuam lub neej ntawm daim nyias nyias. Yog tias tom qab 50 zaug ntawm kev siv, cov dej ntws tsawg dua 50% ntawm cov dej ntws thawj zaug, nws raug nquahu kom hloov cov membrane cassette.
Tom qab tus naj npawb ntawm kev siv mus txog ib qho nqi, yog tias tus qauv rov qab tau qis dua tus nqi teev, nws raug nquahu kom hloov cov membrane cassette.
Dialysis optimization
Yog tias cov txheej txheem cuam tshuam nrog cov kauj ruam dialysis, koj thawj zaug yuav tsum xaiv txoj kev lim ntshav.
Muaj ob hom kev lim ntshav:
Batch dialysis txhais tau hais tias qhov tsis yog ntxiv rau cov qauv tank hauv ib lub ntim, thiab thaum tus qauv yog concentrated rau ib lub ntim, qhov tsis yog ntxiv dua thiab rov ua dua kom txog rau thaum cov kev cai lim ntshav tau ua tiav.
Isovolumetric dialysis txhais tau hais tias nyob rau hauv tus txheej txheem ntawm ultrafiltration, tus nqi ntawm cov outflow ntawm cov permeated kua thiab ntxiv ntawm cov kua tsis yog zoo ib yam, thiab cov reflux kua ntim yog tas li nyob rau hauv tus txheej txheem ntawm isovolumetric dialysis.
Tsis tas li ntawd, tom qab txiav txim siab txoj kev lim ntshav, nws yog ib qho tsim nyog los txiav txim siab seb qhov concentration npaum li cas rau kev lim ntshav. Hauv cov txheej txheem concentration, cov flux feem ntau txo qis thaum cov qauv concentration nce. Dialysis ntawm tsawg concentrations yuav tau txais ib tug ntau dua flux, tab sis lub dialysis ntim yuav loj dua. Thaum kev lim ntshav tau ua tiav ntawm qhov siab dhau lawm, qhov tsis muaj peev xwm yuav cawm tau, tab sis cov flux tsawg dhau. Yog li ntawd, kev lim ntshav yuav tsum tau ua nyob rau ntawm qhov tsim nyog concentration kom optimize lub membrane cheeb tsam los yog txheej txheem lub sij hawm.
Nyob rau hauv tus txheej txheem siab tswj tangential flow ultrafiltration txheej txheem, lub membrane flux thiab cov khoom concentration yog mapped. Thaum pib, lub membrane flux yuav maj mam txo nrog qhov nce ntawm cov khoom concentration. Thaum muaj qhov inflection point, lub membrane flux yuav sai sai txo nrog qhov nce ntawm cov khoom concentration. Lub inflection point sib raug rau cov khoom concentration yog qhov zoo tshaj plaws dialysis concentration, thiab lub membrane cheeb tsam yuav tsum tau rau dialysis yog qhov tsawg tshaj plaws thaum lub sij hawm txheej txheem yog tsau. Txij li thaum nws muaj peev xwm ua tau rau tib lub concentration ntawm cov kuab tshuaj kom muaj qhov sib txawv loj ntawm cov nqi flux hauv cov buffers sib txawv, nws feem ntau yog npaj sib cais rau ob qhov sib txawv tsis sib xws. Txij li kev lim ntshav yog cov txheej txheem ntawm kev hloov pauv ntawm qhov tsis sib xws, qhov kev ua kom zoo tshaj plaws yog feem ntau nyob nruab nrab ntawm ob (piv txwv li, AB hauv daim duab), thiab vim li cas kev nyab xeeb, qhov taw tes nrog qis concentration feem ntau yog xaiv los ua qhov zoo tshaj plaws rau kev lim ntshav. Optimizing lub dialysis point tsuas yog lub theoretical point kom tau qhov tsawg kawg nkaus daim nyias nyias cheeb tsam, uas yuav tsum tau kho raws li qhov teeb meem nyob rau hauv cov txheej txheem tiag tiag. Piv txwv li, thaum optimizing lub dialysis point, lub ntim ntawm cov kua rov qab yog tsawg tshaj li qhov tsawg kawg nkaus ncig ntim los yog cov khoom yog deactivated nyob rau hauv lub sij hawm no, lub concentration ntawm lub dialysis point yuav tsum tau txo.
Tsab ntawv xov xwm no tau coj koj los nkag siab txog cov txheej txheem kev ua haujlwm thiab kev txiav txim siab ntawm ultrafiltration membrane cassette los ntawm ntau yam, thiab muaj ntau yam kev paub txog ultrafiltration membrane cassette, xws li kev ua haujlwm kub zoo tshaj plaws, pH, thiab lwm yam., xyuam xim rau Guidling, thiab yuav coj koj paub ntau ntxiv thiab cov ntaub ntawv yav tom ntej.
Hais txog Guidling
Guidling Technology yog lub teb chaws high-tech enterprise tsom mus rau biopharmaceuticals, cell culture, purification thiab concentration ntawm biomedicine, kuaj mob thiab muaj kua. Peb tau ua tiav cov khoom siv lim dej centrifugal, ultrafiltration & microfiltration cassettes, cov kab mob lim, TFF system, qhov tob lim, hollow fiber, thiab lwm yam. Uas tau raws li daim ntawv thov scenarios ntawm biopharmaceuticals, kab lis kev cai ntawm tes, thiab lwm yam. Peb cov membranes thiab membrane lim tau dav siv nyob rau hauv concentration, extraction thiab sib cais ntawm pre-filtration, microfiltration, ultrafiltration thiab nanofiltration. Peb cov kab ntau cov khoom, los ntawm me me, ib leeg-siv chav kuaj pom mus rau ntau lawm filtration systems, sterility kuaj, fermentation, cell kab lis kev cai thiab lwm yam, ua tau raws li cov kev xav tau ntawm kev sim thiab ntau lawm. Guidling Technology tab tom tos ntsoov rau kev koom tes nrog koj!